Diatheva/Recombinant Listeriolysin-O PEST minus/100 µg/REP0022,Diatheva,,Diatheva,+,,Array.Array.Array蚂蚁淘厂家直采.

Description

Recombinant Listeriolysin O without PEST sequence and with haemolytic activity. The secreted pore-forming protein Listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. PEST sequence is 19 amino acids peptide located at the protein NH 2-terminus, that targets the toxin for degradation. This motif is essential for bacterial virulence.

Additional information

Size	100 µg
Application	Clinical diagnostics\| Veterinary diagnostics\| Life Sciences
Product type	Reagents for research
Reagent type	Recombinant protein
Category	Listeria monocytogenes
Subcategory	Listeriolysin-O

Technical information

Accession number	GenBank AF253320.1 lacking the secretion signal peptide (1-25aa) and PEST sequence (59-529)
Organism	L. monocytogenes
Size	100 µg
Potency	Biologically active protein
Expression system	E.coli
Tag	No tags
Purity	>90%
Form	Liquid, 50mM NaH2PO4, 0.5M NaCl, 2.7mM KCl, 1mM EDTA, 1mM DTT, 5%(v/v) glycerol.
Tested application	SDS-PAGE,
Shipping/storage conditions	Shipped in dry ice . Theprotein is stable for 12 months if stored at -80°C. Avoid freeze-thaw cycles.
Usage	For Laboratory Research Use Only

Download

UniProt database

Datasheet

References

A Novel Role of Listeria monocytogenes Membrane Vesicles in Inhibition of Autophagy and Cell Death

PUBMED ID: 28516064

Related products

Recombinant Listeriolysin-O (LLO) Recombinant Listeriolysin O (LLO) is a protein produced in E. coli. LLO is a single polypeptide protein composed of 529 residues lacking the secretion signal peptide (1-25 aa).	REP0007
Rabbit Anti Listeriolysin O Cholesterol-dependent cytolysins (CDCs) are produced by a large number of pathogenic gram–positive bacteria. A member of this family, listeriolysin O (LLO), is produced by the intracellular pathogen Listeria monocytogenes. Polyclonal anti Listeriolysin antibody is designed for the detection of LLO in in infected speciment as an useful tool in the research of Listeria monocytogenes infections.	ANT0006
Rabbit Anti Listeriolysin O affinity purified Cholesterol-dependent cytolysins (CDCs) are produced by a large number of pathogenic gram–positive bacteria. A member of this family, listeriolysin O (LLO), is produced by the intracellular pathogen Listeria monocytogenes. Polyclonal anti Listeriolysin antibody affinity purified is designed for the specific detection of LLO in in infected speciment as an useful tool in the research of Listeria monocytogenes infections.The affinity purification protocol garantees an improvement on the specificsignal	ANT0016